As I said already in prior post, the main part of watershed segmentation is sequential homotopic thinning transformation, “invented” by a group of mathematicians, including F. Meyer (1976), Ch. Lantuejoul (1978), and J.Serra, from Center for Mathematical Morphology, Fontainebleau, France, and published many times, including different modifications and implementations, such as “SKIZ”, watersheds by flooding, watersheds by hierarchical queues and markers-controlled watersheds. Present modification (see Fig.1 below) was created for for one particular group of Cold Spring Harbor Laboratory (Long Island, NY). Described implementation is based on MAMBA-Image library, developed by S. and N. Beusher.
Fig. 1: Block-diagram of developed segmentation procedure.